ML042-Himedia 10X Tris-Glycine-SDS Gel Running Buffer 10X Tris-Glycine-SDS Gel Running Buffer is the most commonly used as the electrophoresis buffer during the stacking and resolve process of sodium dodecyl sulfate - polyacrylamide gel electrophoresis(SDS-PAGE). This buffer is used as both anode and cathode buffer. This buffer is supplied as 10Xconcentrated stock solution and should be diluted to 1X with deionized water before usage.